![]() Applied Biochemistry and Biotechnology, 162(6), 1751–1761.īenedini, L. Metabolic effects of the initial glucose concentration on microbial production of hyaluronic acid. Applied Biochemistry and Biotechnology, 173(1), 129–142. Fe 2+ and Cu 2+ increase the production of hyaluronic acid by Lactobacilli via affecting different stages of the pentose phosphate pathyway. Applied Biochemistry and Biotechnology, 169(1), 239–249.Ĭhoi, S. Preparation of hyaluronic acid micro-hydrogel by biotin-avidin-specific bonding for doxorubicin-targeted delivery. Applied Biochemistry and Biotechnology, 172(2), 933–950.Ĭui, Y., Li, Y., Duan, Q., & Kakuchi, T. Exopolysaccharides and antimicrobial biosurfactants produced by Paenibacillus macerans TKU029. Hyaluronic acid: A natural biopolymer with a broad range of biomedical and industrial applications. Kogan, G., Šoltés, L., Stern, R., & Gemeiner, P. Applied Microbiology and Biotechnology, 66(4), 341–351. These findings are relevant to further optimizing the precipitation step, thus minimizing costs in the later stages of HA purification.Ĭhong, B. Therefore, pH and NaCl modulated the precipitation performance of HA. Although slight changes in distribution were observed, the average HA molar mass was not affected by the precipitation strategy, remaining on the order of 10 5 Da. Dynamic light scattering (DLS) spectra showed different particle sizes as a consequence of the changes in the molecular structure of HA, mainly with changes in pH. A single-step precipitation was more attractive than multistep precipitation in terms of recovery and purity as well as decreased use of ethanol. Bio-HA was synthesized by Streptococcus zooepidemicus in a culture medium containing glucose and soy peptones. The effects of ethanol/broth proportions and the number of steps at varying pH in the presence or absence of sodium chloride (NaCl) were studied as precipitation strategies for the recovery and purification of high molar mass bio-hyaluronic acid (Bio-HA).
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